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crop seq v2 vector  (Addgene inc)


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    Structured Review

    Addgene inc crop seq v2 vector
    Crop Seq V2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crop+seq+v2+vector/CROPseq-puro-v2+(Plasmid+%23127458)/bio_rxiv__2025__03__17__643814-235-23-26
    Average 93 stars, based on 20 article reviews
    crop seq v2 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Stochastically Emergent Tumors offer in vivo whole genome interrogation of cancer evolution from non-malignant precursors
    Article Snippet: .. The cells were then barcoded using an approach adapted from the ClonMapper system , in which the barcoding plasmid backbone was substituted with CROP-Seq v2 vector (Addgene Plasmid #127458) . ..



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    Journal: iScience

    Article Title: Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

    doi: 10.1016/j.isci.2024.110090

    Figure Lengend Snippet:

    Article Snippet: Briefly, gRNAs were cloned into the BsmBI-digested (New England BioLabs, R0739S) CROP-seq-opti vector by Gibson Assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, E2621L) and NEB Stable Competent E. coli (New England BioLabs, C3040I) were transformed with the assembled plasmids.

    Techniques: Virus, Recombinant, Membrane, Lysis, CRISPR, DNA Extraction, Western Blot, Transfection, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Stripping, Software, Microscopy